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Reference Data

Sample data from Held et al. (2010) shows Hela Kyoto cells stably expressing different combinations of fluorescent markers. 

Full documentation of reagents and experimental details provided in Held et al. (2010).

Chromatin + Microtubles

H2B-mCherry (red) + mEGFP-α-Tubulin (green)

Human Hela Kyoto cells stably expressing fluorescent markers of chromatin (histone-2b, H2B) and alpha-Tubulin.

Data acquired by Katja Beck (Kutay group, ETH Zurich) and Michael Held (Gerlich group, ETH Zurich) at a Molecular Devices ImageXpress Micro with the PlateScanPackage.

Widefield epifluorescence, 20x dry objective (0.3225 µm / pixel), 1392x1040 pixel, 206 frames x 4.6 min = 15.8 h

H2b_aTub_MD20x_exp911_P0037_small.jpg

Overlay movies
  • Movie (MOV h264, 50% resolution, 18.6 MB)
  • Movie (MOV h264, 100% resolution, 138.4 MB)
Raw data
  • Images (ZIP, reduced set for classifier retraining, 3 positions, 899.6 MB)
  • Images (ZIP, extended set, 9 positions, 2.6 GB)
Analysis data
  • Analysis (TAR.BZ2, features, tracking data, events, w/o visualization images, 445.2 MB)
  • Analysis (TAR.BZ2, features, tracking data, events, w/ visualization images, 11.1 GB)
Classifier data
  • H2B (ZIP, annotated feature, libSVM model, w/ sample visualization, 4.1 MB)
  • a-Tubulin (ZIP, annotated feature, libSVM model, w/ sample visualization, 2.7 MB)

 

Chromatin + Golgi aparatus

H2B-mCherry (red) + GalT-GFP (green)

Human Hela Kyoto cells stably expressing fluorescent markers of chromatin (histone-2b, H2B) and the Golgi aparatus (Galactosyl transferase, GalT).

Data acquired by Michael Schmitz (Gerlich group, ETH Zurich) at a Molecular Devices ImageXpress Micro with the PlateScanPackage.

Widefield epifluorescence, 10x dry objective (0.645 µm / pixel), 1392x1040 pixel, 482 frames x 2.8 min = 22.5 h

H2b_GalT_MD10x_exp835_P0023_small.jpg

Overlay movies
  • Movie (MOV h264, 50% resolution, 30.4 MB)
  • Movie (MOV h264, 100% resolution, 252.5 MB)
Raw data
  • Images (ZIP, reduced set for classifier retraining, 1 position, 723.0 MB)
  • Images (ZIP, extended set, 6 positions, 4.0 GB)
Analysis data
  • Analysis (TAR.BZ2, features, tracking data, events, w/ visualization images, 18.6 MB)
  • Analysis (TAR.BZ2, features, tracking data, events, w/o visualization images, 18.6 MB)
Classifier data
  • H2B (ZIP, annotated feature, libSVM model, w/ sample visualization, 2.8 MB)
  • GalT (ZIP, annotated feature, libSVM model, w/ sample visualization, 2.8 MB)


Chromatin + DNA replication factories

H2B-mCherry (red) + EGFP-PCNA (green)

Human Hela Kyoto cells stably expressing fluorescent markers of chromatin (histone-2b, H2B) and DNA replication factories (proliferation cell nuclear antigen, PCNA).

Data acquired by Michael Olma (Peter group, ETH Zurich) at a Molecular Devices ImageXpress Micro with the PlateScanPackage.

Widefield epifluorescence, 10x dry objective (0.645 µm / pixel), 1392x1040 pixel, 482 frames x 5.9 min = 47.4 h

H2b_PCNA_MD10x_MOexp90_P0015_small.jpg

Overlay movies

  • Movie (MOV h264, 50% resolution, 12.9 MB)
  • Movie (MOV h264, 100% resolution, 163.9 MB)
Raw data
  • Images (ZIP, reduced set for classifier retraining, 1 position, 437.5 GB)
  • Images (ZIP, extended set, 4 positions, 3.4 GB)
Analysis data
  • Analysis (TAR.BZ2, features, tracking data, events, w/o visualization images, 848.7 MB)
  • Analysis (TAR.BZ2, features, tracking data, events, w/ visualization images, 11.4 GB)
Classifier data
  • H2B (ZIP, annotated feature, libSVM model, w/ sample visualization, 4.9 MB)
  • PCNA (ZIP, annotated feature, libSVM model, w/ sample visualization, 4.8 MB)
 

Chromatin + Nuclear import substrate

H2B-RFP (red) + IBB-mEGFP (green)

Human Hela Kyoto cells stably expressing fluorescent markers of chromatin (histone-2b, H2B) and the nuclear import substrate (importin-beta binding domain of importin-alpha, IBB).

Data acquired by Michael Schmitz (Gerlich group, ETH Zurich) at a Molecular Devices ImageXpress Micro with the PlateScanPackage.

Widefield epifluorescence, 10x dry objective (0.645 µm / pixel), 1392x1040 pixel, 744 frames x 3.7 min = 45.9 h

H2b_IBB_MD10x_exp665_P0028_small.jpg

Overlay movies
  • Movie (MOV h264, 50% resolution, 24.4 MB)
  • Movie (MOV h264, 100% resolution, 231.8 MB)
Raw data
  • Images (ZIP, reduced set for classifier retraining, 4 positions, 1.6 GB)
  • Images (ZIP, extended set, 6 positions, 2.5 GB)
Analysis data
  • Analysis (TAR.BZ2, features, tracking data, events, w/o visualization images, 490.4 MB)
  • Analysis (TAR.BZ2, features, tracking data, events, w/ visualization images, 11.7 GB)
Classifier data
  • H2B (ZIP, annotated feature, libSVM model, w/ sample visualization, 4.9 MB)

 

 

 

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